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Antibodies sure to the blocking peptide no more bind towards the epitope over the focus on protein. By comparing the staining with the blocked antibody compared to the antibody by itself, one can see which staining is unique.
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This guide outlines even more possible optimization of cellular and immunoassay parameters to be certain the best possible effects are...
This manual outlines additional achievable optimization of mobile and immunoassay parameters to make certain the very best results are...
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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Blocking peptides are peptides that bind especially for the goal antibody and block antibody binding. These peptide generally consists of the epitope recognized from the antibody.
Target Information and facts Cyclin E belongs on the very conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins function as regulators of CDK kinases. Distinct cyclins show distinctive expression and degradation styles which contribute towards the temporal coordination of each and every mitotic function. Cyclin E kinds a fancy with and features being a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S period boundary which is degraded as cells progress by way of S section.
Overexpression of this gene has been observed in several tumors, which results in chromosome instability, and therefore may perhaps add to tumorigenesis. This protein was found to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a vital function in promoting cell-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have already been described. Two supplemental splice variants have been described but in-depth nucleotide sequence info is just not still readily available.
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